Analysis of drug-resistant multi-loci mutation of P area of HBV genome in 32 patients with chronic hepatitis B / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>OBJECTIVE</b>In order to deeply understand the effects of nucleoside analogues to HBV DNA polymerase area, which initiate a state of drug resistance due to HBV genome mutation.</p><p><b>METHODS</b>Using PCR product direct sequencing method to test the gene sequence of P area of HBV genome of the patients who taking nucleoside analogues and showing signs of virologic breakthrough.</p><p><b>RESULTS</b>More resistance mutations can be find in HBV DNA polymerase area, which causing clinically cross drug resistance and multiple resistance.</p><p><b>CONCLUSION</b>We should choose higher genetic barrier for the initial treatment. And the patients long-term using of nucleoside analogues, should be regularly monitored serum HBV DNA level, in order for early detection of virological breakthrough and timely intervention.</p>