Inhibitory effect of transforming growth factor-β(1) on oral squamous cell carcinoma brain metastasis Tb cell line / 中华口腔医学杂志
Chinese Journal of Stomatology
; (12): 421-425, 2010.
Article
in Zh
| WPRIM
| ID: wpr-243159
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the inhibitory effect of transforming growth factor (TGF)-β₁ on oral squamous cell carcinoma (OSCC) Tb cell line.</p><p><b>METHODS</b>Cell counting method was used to examine the inhibitory effect of TGF-β₁ on Tb cell and flow cytometry (FCM) assay performed to measure the changes of cell cycle. Superarray was used to screen the changing expression of genes in TGF-β₁/Smads signaling pathway.RT-PCR method was used to detect the results of Superarray.</p><p><b>RESULTS</b>TGF-β₁ showed significant inhibiting effect on OSCC Tb cell line. TGF-β₁ blocked the cell cycle at G₁ phase. The expression level of activin receptor-like kinase-1 (ACVRL-1), anti-mullerian hirmine (AMH), cyclim-dependent kinase inhibitor-2B (CDKN-2B) and transforming growth factor-beta-indnced factor (TGIF) was higer in the cells treated with TGF-β₁ than in control, while TDGF-1 expression was down-regulated. ACVRL-1 and CDKN-2B gene expression was consistent with the results of Superarray.</p><p><b>CONCLUSIONS</b>TGF-β₁ can inhibit the growth of OSCC Tb cell line. The mechanism may be related to the regulation of cell cycle and the expression of ACVRL-1 and CDKN-2B in TGF-β₁-Smads signaling pathway.</p>
Full text:
1
Database:
WPRIM
Main subject:
Pathology
/
Pharmacology
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Carcinoma, Squamous Cell
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Signal Transduction
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Activin Receptors, Type II
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Cell Line, Tumor
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Cyclin-Dependent Kinase Inhibitor p15
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Transforming Growth Factor beta1
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Anti-Mullerian Hormone
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Cell Cycle Checkpoints
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Stomatology
Year:
2010
Document type:
Article