Transcription Factors Regulating Inflammatory Cytokine Production Are Differentially Expressed in Peripheral Blood Mononuclear Cells of Behçet Disease Depending on Disease Activity
Annals of Dermatology
; : 173-179, 2017.
Article
in En
| WPRIM
| ID: wpr-25589
Responsible library:
WPRO
ABSTRACT
BACKGROUND:
Behçet disease (BD) is a relapsing inflammatory disease with increased production of inflammatory cytokines in peripheral blood mononuclear cells (PBMCs); however, the underlying molecular mechanisms are not well known.OBJECTIVE:
To analyze whether the differential expression of transcription factors is involved in the increased tumor necrosis factor (TNF)-α and interleukin (IL)-6 production by PBMCs of BD patients compared to healthy controls (HCs).METHODS:
Expression of transcription factors was examined by real-time reverse transcriptase-polymerase chain reaction and western blotting. Cytokine production by CD11b+ cells transfected with siRNAs against transcription factors was measured by enzyme-linked immunosorbent assay.RESULTS:
In the absence of lipopolysaccharide stimulation, the transcript level of CCAAT-enhancer-binding proteins (C/EBP) β was increased in PBMCs from patients with active BD compared to that in PBMCs from patients with stable BD. The C/EBPδ transcript level was higher in PBMCs from patients with active BD than in those from HCs. The activating transcription factor 3 (ATF3) transcript level was increased in PBMCs from patients with stable BD compared to that in PBMCs from HCs. siRNAs targeting C/EBPβ and C/EBPδ significantly reduced the production of IL-6 and TNF-α in lipopolysaccharide-stimulated CD11b+ cells from patients with BD as well as from HCs.CONCLUSION:
We found differential expression of C/EBPβ, C/EBPδ, and ATF3 in PBMCs from patients with BD depending on disease activity, indicating the involvement of these molecules in BD pathogenesis.Key words
Full text:
1
Database:
WPRIM
Main subject:
Transcription Factors
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Enzyme-Linked Immunosorbent Assay
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Gene Expression
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Blotting, Western
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Behcet Syndrome
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Cytokines
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Interleukins
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Interleukin-6
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Tumor Necrosis Factor-alpha
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CCAAT-Enhancer-Binding Proteins
Limits:
Humans
Language:
En
Journal:
Annals of Dermatology
Year:
2017
Document type:
Article