A PCR-based Rapid Neutralization Assay for GII.4 Norovirus Infection in HIEC6 Cell Culture / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 219-221, 2015.
Article
in En
| WPRIM
| ID: wpr-264597
Responsible library:
WPRO
ABSTRACT
Because of limited viral replication and lack of cytopathic effect in cell culture, a new PCR-based rapid seroneutralization assay for detection of GII.4 norovirus neutralized antibodies was developed with serum samples from acute-phase patients, convalescent-phase patients and healthy controls. According to this study, neutralizing antibodies were detected in 100% of convalescent-phase sera, and in 2.5% of healthy controls sera. However, all of the acute-phase serum samples could not neutralize virus efficiently. Compared to the results from ELISA (96.2% at sensitivity and 80% at specificity), the present in vitro neutralization assay is more specific and more sensitive.
Full text:
1
Database:
WPRIM
Main subject:
Virology
/
Enzyme-Linked Immunosorbent Assay
/
Base Sequence
/
Case-Control Studies
/
Cell Line
/
Polymerase Chain Reaction
/
Sensitivity and Specificity
/
DNA Primers
/
Caliciviridae Infections
/
Norovirus
Type of study:
Diagnostic_studies
/
Observational_studies
Limits:
Humans
Language:
En
Journal:
Biomedical and Environmental Sciences
Year:
2015
Document type:
Article