Microbial transformation of glycyrrhetinic acid by Cunninghamella blakesleeana / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 4212-4217, 2015.
Article
in Zh
| WPRIM
| ID: wpr-279259
Responsible library:
WPRO
ABSTRACT
A study on the microbial transformation of glycyrrhetinic acid (GA) was conducted by a fungus, Cunninghamella blakesleeana CGMCC 3.970 systematically. After incubation with the cell cultures of C. blakesleeana CGMCC 3.970 at 25 degrees C for 7 days on a rotary shaker operating at 135 r x min(-1), GA was converted into one major product and five minor products. The products were extracted and purified by solvent extraction, macroporous adsorbent resin, silica gel column chromatography, and semi-preparative RP-HPLC chromatography. Their structures were identified as 3-oxo-15α-hydroxy-18β-glycyrrhetinic acid(1), 3-oxo-15β-hydroxy-18β-glycyrrhetinic acid (2), 7β,15α-dihydroxy-18β-glycyrrhetinic acid (3), 3-oxo-7β, 15α-dihydroxy-18β-glycyrrhetinic acid (4), 7β-hydroxy-18β-glycyrrhetinic acid(5) and 15α-hydroxy-18β-glycyrrhetinic acid(6) by the analyses of MS, 1H-NMR and 13C-NMR spectroscopic data respectively. Among them, 2 was a new compound. These results suggest that C. blakesleeana CGMCC 3.970 has the capability of selective ketonization and hydroxylation for GA. [Key words] glycyrrhetinic acid; Cunninghamella blakesleeana CGMCC 3. 970; microbial transformation
Full text:
1
Database:
WPRIM
Main subject:
Biotransformation
/
Molecular Structure
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Chemistry
/
Cunninghamella
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Spectrometry, Mass, Electrospray Ionization
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Glycyrrhetinic Acid
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Metabolism
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2015
Document type:
Article