Gaussia luciferase reporter assay for assessment of gene delivery systems in vivo / 中国医学科学杂志(英文版)
Chinese Medical Sciences Journal
; (4): 95-99, 2010.
Article
in En
| WPRIM
| ID: wpr-299451
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To develop an alternative method for assessment of gene delivery systems in vivo.</p><p><b>METHODS</b>Mouse primary spleen lymphocytes were genetically modified in vitro by a retroviral vector harboring a Gaussia luciferase (Gluc) expression cassette. After implantation of these cells into recipient mice, the expression of Gluc was detected in whole blood or plasma collected.</p><p><b>RESULTS</b>As little as 10 muL whole blood drawn from the recipient mice could guarantee prompt reading of Gluc activity with a luminometer. And the reading was found in good correlation with the number of genetically modified spleen lymphocytes implanted to the mice.</p><p><b>CONCLUSIONS</b>Gluc may be useful as an in vivo reporter for gene therapy researches, and Gluc blood assay could provide an alternative method for assessment of gene delivery systems in vivo.</p>
Full text:
1
Database:
WPRIM
Main subject:
Cell Line
/
Genes, Reporter
/
Gene Transfer Techniques
/
Arecaceae
/
Genetics
/
Luciferases
Limits:
Animals
/
Humans
Language:
En
Journal:
Chinese Medical Sciences Journal
Year:
2010
Document type:
Article