Prediction and research on homology of B-cell epitopes of Epstein-Barr virus nuclear antigen-1 / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 371-375, 2011.
Article
in Zh
| WPRIM
| ID: wpr-306557
Responsible library:
WPRO
ABSTRACT
We predict in this paper B-cell epitopes of Epstein-Barr virus nuclear antigen-1 (EBNA-1) and analyze the results matched with the related autoantigens sequence of human. We selected EBV-1 standard strain NA-1 amino acid sequence as the basis. We predicted B-cell dominant epitopes of EBNA-1 with the methods of SOPMA, GOR and HNN, combined with the multi-parameter analysis of transmembrane domain, hydrophilicity profile, surface probability, antigenicity index, polarity and average flexibility. The blastp method was adopted to analyze the matched results between the predicted B-cell epitopes of EBNA-1 and the related autoantigens sequence of human. The results have shown that the possible B-cell dominant epitopes of EBNA-1 were located in the N terminal regions of 16-23, 35-78, 332-337, 340-357, 398-404, 419-432 and 620-637, in which different regions gained higher scores when matched with small nuclear ribonucleoprotein SmB, SmD, ribonucleoprotein SSA, heterogeneous nuclear ribonucleoprotein hnRNP A1, hnRNP G, respectively. It was available to predict B-cell dominant epitopes of EBNA-1 with multiparameter methods and to analyze the same or similar autoantigens sequences of human, which laid a theory foundation for the study of pathogenesis, diagnosis and treatment of autoimmune diseases.
Full text:
1
Database:
WPRIM
Main subject:
Autoantigens
/
Molecular Sequence Data
/
Base Sequence
/
Amino Acid Sequence
/
Sequence Homology, Amino Acid
/
Epitopes, B-Lymphocyte
/
Epstein-Barr Virus Nuclear Antigens
/
Allergy and Immunology
Type of study:
Prognostic_studies
Limits:
Humans
Language:
Zh
Journal:
Journal of Biomedical Engineering
Year:
2011
Document type:
Article