SLAM family predicting the initiation potential of human acute lymphoblastic leukemia in NOD/SCID mice / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 3074-3079, 2011.
Article
in En
| WPRIM
| ID: wpr-319194
Responsible library:
WPRO
ABSTRACT
<p><b>BACKGROUND</b>The SLAM family recently has been reported to show an important biological role in lymphocyte development and immunological function, and it is efficient to highly purify hematopoietic stem cells using a simple combination of SLAM family members. To elucidate the presence of this family on acute lymphoblastic leukemia (ALL), as well as its relationship with the leukemia-initiating potential, we analyzed the expression pattern of this family members on human ALL progenitor cells, combined with serial xenotransplantation assay.</p><p><b>METHODS</b>Expression analysis was carried out by flow cytometry. We combined the expression pattern of human CD(150), CD(244) and CD(48) with serial xenotransplantation of B-ALL progenitor cells to indicate their relationship.</p><p><b>RESULTS</b>CD(48) and CD(244) were expressed on most B-ALL progenitor cells, the percentage being (93.08 ± 6.46)% and (63.37 ± 29.31)%, respectively. Interestingly, the proportion of CD(150)(+) cells declined obviously in engrafted cases ((24.94 ± 7.32)%) compared with non-engrafted cases ((77.54 ± 5.93)%, P < 0.01), which indicated that only blast cells with low percentage of CD(150)(+) population were able to reconstitute leukemia into primary, secondary and tertiary NOD/SCID mice.</p><p><b>CONCLUSIONS</b>SLAM family members are present on B-ALL progenitor cells and the leukemia-initiating potential of leukemic blasts is correlated negatively with the proportion of CD(150)(+) cells, the percentage of which can serve as a useful predictor for engraftment success of B-ALL to immune deficient mice.</p>
Full text:
1
Database:
WPRIM
Main subject:
Transplantation, Heterologous
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Blood
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Receptors, Immunologic
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Antigens, CD
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Mice, SCID
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Mice, Inbred NOD
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Receptors, Cell Surface
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Precursor Cell Lymphoblastic Leukemia-Lymphoma
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Flow Cytometry
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CD48 Antigen
Type of study:
Prognostic_studies
Limits:
Animals
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Female
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Humans
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Male
Language:
En
Journal:
Chinese Medical Journal
Year:
2011
Document type:
Article