Construction of infectious Japanese encephalitis virus clone based on the cDNA template of the attenuated live vaccine production strain SA14-14-2 / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 9-11, 2005.
Article
in Zh
| WPRIM
| ID: wpr-333064
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To construct infectious Japanese encephalitis virus (JEV) based on the in vitro-ligated cDNA template of the vaccine strain SA14-14-2, and identify the virus.</p><p><b>METHODS</b>Full-length genomic cDNA of JEV SA14-14-2 strain was ligated and then RNA was transcribed in vitro, the infective virus was obtained by transfecting the RNA into Vero cells and identified.</p><p><b>RESULTS</b>The infective clone of JEV was constructed, the virulence was weaker than the wild virus.</p><p><b>CONCLUSION</b>It was possible to construct infectious clone from the production strain of live attenuated Japanese B encephalitis vaccine.</p>
Full text:
1
Database:
WPRIM
Main subject:
Pathology
/
Virology
/
Virulence
/
Vero Cells
/
RNA, Viral
/
Vaccines, Attenuated
/
Base Sequence
/
Cells, Cultured
/
Chlorocebus aethiops
/
Genome, Viral
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2005
Document type:
Article