Circulating microRNA expression in children with idiopathic short stature / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
; (12): 1104-1108, 2013.
Article
in Zh
| WPRIM
| ID: wpr-345636
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the role of circulating microRNAs (miRNA) in the pathogenesis of idiopathic short stature (ISS) through detecting miRNA expression profile in plasma of children with ISS.</p><p><b>METHODS</b>Plasma miRNA expression was determined by microarray in 20 children with ISS and 20 healthy children. Altered microRNAs were verified by real-time PCR. The online miRNA target gene prediction software was used to predict and screen miRNA differentially expressed target genes.</p><p><b>RESULTS</b>According to the microarray, there were 40 differentially expressed miRNAs in the ISS group compared with the control group, including 24 up-regulated miRNAs and 16 down-regulated miRNAs. Real-time PCR verified two up-regulated (miR-185and miR-574-5p) and two down-regulated miRNAs (miR-497and miR-15a) and confirmed that plasma miR-185 expression was significantly up-regulated (P<0.05) and miR-497 expression was significantly down-regulated (P<0.05) in children with ISS.</p><p><b>CONCLUSIONS</b>Plasma miRNA expression levels in children with ISS are significantly different from healthy controls, suggesting that plasma miRNA is associated with the pathogenesis of ISS.</p>
Full text:
1
Database:
WPRIM
Main subject:
Blood
/
Oligonucleotide Array Sequence Analysis
/
MicroRNAs
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Real-Time Polymerase Chain Reaction
/
Genetics
/
Growth Disorders
Limits:
Child
/
Female
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Humans
/
Male
Language:
Zh
Journal:
Chinese Journal of Contemporary Pediatrics
Year:
2013
Document type:
Article