The role of vimentin during PRRSV infection of Marc-145 cells / 病毒学报
Chinese Journal of Virology
; (6): 456-461, 2011.
Article
in Zh
| WPRIM
| ID: wpr-354806
Responsible library:
WPRO
ABSTRACT
The objective of this study was to investigate the function of vimentin in PRRSV infection. Vimentin gene from Marc-145 cells was amplified by RT-PCR, cloned into pET-28a vector and expressed in Escherichia coli BL21(DE3). The expressed vimentin was confirmed by Western blot and purified which was used to immunize BALB/c mice for the production of antibodies. Vimentin and antibodies were tested for blocking PRRSV infection of Marc-145 cells. The binding of vimentin to PRRSV N and GP5 proteins were tested by the ELISA. The results showed that vimentin gene was amplified successfully and expressed as identified by SDS-PAGE and Western blot. Mouse anti-vimentin antibodies were produced with the titer of 10(5). PRRSV infection of Marc-145 cells was blocked partially by vimentin while blocked completely by the antibobies. In addition, vimentin was bound N protein, but not GP5. These results provide additional information on PRRSV entry into Marc-145 cells.
Full text:
1
Database:
WPRIM
Main subject:
Physiology
/
Protein Binding
/
Swine
/
Vimentin
/
Viral Proteins
/
Virology
/
Recombinant Proteins
/
Cell Line
/
Porcine respiratory and reproductive syndrome virus
/
Porcine Reproductive and Respiratory Syndrome
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Virology
Year:
2011
Document type:
Article