Biological transport of tetracycline hydrochloride by human periodontal ligament fibroblasts / 中华口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate biological transport of tetracycline hydrochloride by human periodontal ligament fibroblasts (HPDLF) for verifying the hypothesis of delivering medicine to the periodontium and whole body through the root canal.</p><p><b>METHODS</b>HPDLF and MC3T3-E1 cells were incubated in antibiotics solutions. The intracellular antibiotics contents were measured by high performance liquid chromatography (HPLC) and the cell total protein was measured by bradford protein assay.</p><p><b>RESULTS</b>The intracellular contents increased with incubation time. The extracellular medicine concentration had effect on the intracellular contents.</p><p><b>CONCLUSIONS</b>Tetracycline hydrochloride can be transported into HPDLF with incubation and this transport is time-dependent and concentration-dependent.</p>