Effect of platelet-rich plasma on human periodontal ligament fibroblasts' proliferation, migration and differentiation / 实用口腔医学杂志
Journal of Practical Stomatology
; (6): 194-197, 2010.
Article
in Zh
| WPRIM
| ID: wpr-403429
Responsible library:
WPRO
ABSTRACT
Objective:
To investigate the in vitro effects of platelet-rich plasma(PRP) on human periodontal ligament fibroblasts(PDLFs).Methods:
Various concentrations of PRP (10, 50, 100, 200, 300, 500 ml/L) were applied to primary cultures of human PDLFs. MTT assays were utilized to assess cell proliferation ability. Migration was determined by assessing the cell response to a concentration gradient with Transwell chamber. Differentiation was assessed using alkaline phosphatase (ALP) kit.Results:
A beneficial effect on proliferation was observed, especially in response to 200 ml/L PRP.PRP had stimulatory effects on the migration of human PDLFs. PRP facilitated differentiation of PDLFs.Conclusion:
PRP can exert a positive effect on human PDLFs,but this effect is concentration specific, while higher concentrations is not necessary to result in optimal outcomes.
Full text:
1
Database:
WPRIM
Language:
Zh
Journal:
Journal of Practical Stomatology
Year:
2010
Document type:
Article