In Vitro and In Vivo Imaging of Prostate Cancer Angiogenesis Using Anti-Vascular Endothelial Growth Factor Receptor 2 Antibody-Conjugated Quantum Dot
Korean Journal of Radiology
; : 30-37, 2013.
Article
in En
| WPRIM
| ID: wpr-44598
Responsible library:
WPRO
ABSTRACT
OBJECTIVE: Authors aimed to determine the targeting ability of vascular endothelial growth factor receptor 2 (VEGFR2)-conjugated quantum dots (QDs) in vitro, and apply it for a xenograft prostate cancer mouse model. MATERIALS AND METHODS: Conjugation reaction of QDs was performed by using the N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide (EDC) and sulfo-(N-hydroxysulfosuccinimide) (Sulfo-NHS). The human umbilical vein cord endothelial cells (HUVECs) were incubated with QDs, conjugated with antiVGFR2, to see a specific binding in vitro. Fluorescent cell images were taken by a confocal microscope. The human prostate cancer cells (PC3) were injected to five nude mice on hind limbs to make the xenograft tumor model. QD-antiVEGFR2 antibody complex was injected into the tumor model and fluorescence measurements were performed at 1, 4, 9, 12, 15, and 24 hours after the injection. RESULTS: The specific interaction between HUVECs and QD-antiVEGFR2 antibody was clearly shown in vitro. The in vivo fluorescence image disclosed that there was an increased signal of tumor, 12 hours after the injection of QDs. CONCLUSION: By showing endothelial cells binding with QDs-antiVEGFR2 antibodyand an experimental application of the antibody for VEGFR2 imaging in the prostate cancer xenograft mouse model, we suggests that the antibody-conjugated QDs can be a potential imaging tool for angiogenesis of the cancer.
Key words
Full text:
1
Database:
WPRIM
Main subject:
Prostatic Neoplasms
/
Succinimides
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Transplantation, Heterologous
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Carbodiimides
/
Microscopy, Confocal
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Vascular Endothelial Growth Factor Receptor-2
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Cell Line, Tumor
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Quantum Dots
/
Disease Models, Animal
/
Electrophoresis, Agar Gel
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Korean Journal of Radiology
Year:
2013
Document type:
Article