Tissue engineered cartilage constructed by growth factor-induced bone marrow mesenchymal stem cells and allogenic bone matrix gelatin / 中华骨科杂志

ABSTRACT

Objective To construct tissue engineered cartilage with bone marrow mesenchymal stem cells (MSCs) and allogeneic bone matrix gelatin (BMG). Methods Bone marrow was aspirated and MSCs were separated by consecutive 0.70(70%)(V/V) Percoll gradient centrifugation and fibronectin adhesion. In experimental group, rhTGF-?1 and rhIGF-Ⅰ were applied into DMEM to induce proliferation and chondrogenic transformation, while in control group only DMEM was used. Colony forming efficiency (CFE) of MSCs was calculated, procollagen ?1(Ⅱ)mRNA expression in cells was detected by RT-PCR, hexuronic acid in culture medium was measured by carbazole-sulfuric acid method. Allogeneic bone matrix gelatin (BMG) was prepared by successive defatting and decalcification, observed by scanning electronic microscope. Chondrocyte progenitor cells induced from MSCs were seeded into BMG to construct tissue engineered cartilage. Culture masses were sectioned and stained by Masson's trichrome. Results CFE was 21/106 in experimental group, significantly higher than that of control group, which was 3/106 (u=3.878, P