Effect of 15d-PGJ_2 on proliferation and apoptosis of HSC-T6 / 中国药理学通报
Chinese Pharmacological Bulletin
; (12)1987.
Article
in Zh
| WPRIM
| ID: wpr-560969
Responsible library:
WPRO
ABSTRACT
Aim To evaluate the effect of 15d-PGJ2 on up-regulated expression of PPAR? and inducing HSC apoptosis and inhibiting HSC proliferation. Methods The rat liver stellate cell line (HSC-T6) was cultured in DMEM, and treated with PPAR? agonist 15d-PGJ2 of different concentrations. The expression of PPAR? mRNA was detected by RT-PCR. The protein expression of NF-?B was examined by Western blot. The cell proliferation rate of HSC-T6 was determined by MTT colorimetric assay. The cell cycle and apoptosis ratio were measured using flow cyto -metry analysis. Results The proliferation rate of the rat liver stellate cell line (HSC-T6) was significantly inhibited by 15d-PGJ2 (vs controls, P
Full text:
1
Database:
WPRIM
Language:
Zh
Journal:
Chinese Pharmacological Bulletin
Year:
1987
Document type:
Article