Detecting a novel CXC chemokine macrophage inflammatory protein-2? mRNA by real-time fluorescent quantitative reverse transcription polymerase chain reaction (TaqMan) / 中华检验医学杂志

ABSTRACT

Objectives To develop and evaluate a real-time fluorescent quantitative RT-PCR method for the detection of a novel CXC chemokine macrophage inflammatory protein-2?(MIP-2?)mRNA expression based on TaqMan technique.Method The expression of MIP-2? mRNA was detected by TaqMan real time fluorscet quantitative RT-PCR. Results The dynamic range of the assay varied from 102 to 107 copies. The intra- and inter-assay coefficient variation (CV) were less than