Optical Spectra Diversity and in vitro Molecular Evolution of Red Fluorescent Proteins / 生物化学与生物物理进展

ABSTRACT

Red fluorescent proteins (RFPs) produced from a number of Anthozoa species have been subjected to a series of in vitro molecular evolution, resulting in various emission spectra ranging from 570 nm to 655 nm and thus providing powerful tools for cellular imaging or even body imaging. This article briefly reviewed the optical properties, structures and mutagenesis of RFPs and their applications.