Hydrolysis of Adenylic Acid by Human Skin Tissue in vitro: 5'-Nucleotidase Activity in the Epidermis and Dermis / 대한피부과학회지

ABSTRACT

The incubation of adenosine 5-monophosphate(AMP) with the homogenates of the epidermis and dermis, which were obtained from the axilIary skin of osmidrosis (bromidrosis) patients, resulted in the formation of adenosine and inorganic phosphate (Pi) without further degradation, as demonstrated by paper chromatography. The conversion of AMP to adenosine in the skin was catalyzed by 5-nucleotidase and alkaline phosphatase. It was found that 5-nucleotidase was present both in the epidermis and dermis, being more active in the latter, and that the enzyme was responsible for more than 80% of the total AMP-hydrolyzing activity present in the skin homogenates. Alkaline phosphatase was shown to be present mainIy in the dermis. And its contribution to AMP hydrolysis was insignificant at pH 7.4. From these results, it is evident that AMP is converted to adenosine chiefly by 5-nucleotidase, which is present in the epidermis and dermis.