Expression of GATA-3 in Pulmonary Tissue of Asthmatic Mice and Inhibitory Effect of Dexamethsone on It / 实用儿科临床杂志
Journal of Applied Clinical Pediatrics
; (24)2006.
Article
in Zh
| WPRIM
| ID: wpr-639429
Responsible library:
WPRO
ABSTRACT
Objective To explore the expression of GATA-3 in pulmonary tissue of asthmatic mice and the inhibitory effect of dexamethsone(Dex)on it.Methods The Blab/c mice asthma model was induced by ovalbumin(OVA) with classic method.Twenty-four male mice were randomly divided into control group,asthmatic group and Dex treated group.The expression of GATA-3 protein was measured by immunohistochemical staining.The expression of GATA-3 mRNA was measured by reverse transcription-polymerase chain reaction(RT-PCR).The level of IL-4 in mice spleen CD4 T cell was measured by flow cytometry.The airway inflammation was evaluated by HE staining.Results The percentages of postive GATA-3,GATA-3 mRNA and IL-4 protein of asthmatic group were significantly higher than those of control group(P
Full text:
1
Database:
WPRIM
Language:
Zh
Journal:
Journal of Applied Clinical Pediatrics
Year:
2006
Document type:
Article