Effect of pregnancy-associated plasma protein-A on the function of endothelial cells / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 1261-1265, 2010.
Article
in Zh
| WPRIM
| ID: wpr-814335
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To determine the effect of pregnancy-associated plasma protein A (PAPP-A) on the function of vascular endothelial cells (VEC).@*METHODS@#Human umbilical vein endothelial cell (HUVEC) line, derived from human umbilical vein, was cultured in vitro with PAPP-A at 0, 50, 100, and 200 ng/mL for 0, 12, 24, and 48 hours, respectively. Nitric oxide (NO) levels and endothlin-1 (ET-1) levels were determined by spectrophotometer and immunehistory.@*RESULTS@#The NO levels of HUVECs in the PAPP-A groups were significantly lower than those in the control group (P<0.05). The ET-1 levels of HUVECs in the PAPP-A groups were significantly higher than those in the control group (P<0.05). The changes were all dose-dependent.@*CONCLUSION@#PAPP-A may affect the function of vascular endothelial cells by reducing the secretion of NO and increasing the level of ET-1.
Full text:
1
Database:
WPRIM
Main subject:
Pharmacology
/
Physiology
/
Pregnancy-Associated Plasma Protein-A
/
Umbilical Veins
/
Cell Line
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Endothelin-1
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Cell Biology
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Endothelial Cells
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Metabolism
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Nitric Oxide
Limits:
Female
/
Humans
Language:
Zh
Journal:
Journal of Central South University(Medical Sciences)
Year:
2010
Document type:
Article