Effects of fine Particulate Matter(PM2.5) on the autophagy in human skin melanocytes / 公共卫生与预防医学

ABSTRACT

Objective  To study the effects of fine particulate matter(PM2.5) on the autophagy in human melanocytes. Methods PM2.5 was collected in Huanggang city. Then the water-soluble extracts and non-water-soluble extracts were isolated and the melanocytes were exposed to a W-PM2.5 or NW-PM2.5 with various concentrations. Autophagy of melanocytes was observed by MDC staining, and the expression of LC3-II /I were detected by immunohistochemistry and western blot. Results With the increase of concentration of W-PM2.5 and NW-PM2.5, the expression of melanocyte autophagosome and LC3-II /I increased, and the autophagy response was significantly enhanced（P2.5 components had a stronger effect on promoting LC3II/I expression than W-PM2.5 components（P2.5 can induce oxidative stress injury and excessive autophagy of melanocytes.