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Expression analysis of long non-coding RNAs in a renal ischemia-reperfusion injury model
Tao, Qiu; Tianyu, Wang; Jiangqiao, Zhou; Zhongbao, Chen; Xiaoxiong, Ma; Long, Zhang; Jilin, Zou.
Affiliation
  • Tao, Qiu; Wuhan University. Renmin Hospital. Department of Organ Transplantation. Hubei. CN
  • Tianyu, Wang; Wuhan University. Renmin Hospital. Department of Organ Transplantation. Hubei. CN
  • Jiangqiao, Zhou; Wuhan University. Renmin Hospital. Department of Organ Transplantation. Hubei. CN
  • Zhongbao, Chen; Wuhan University. Renmin Hospital. Department of Organ Transplantation. Hubei. CN
  • Xiaoxiong, Ma; Wuhan University. Renmin Hospital. Department of Organ Transplantation. Hubei. CN
  • Long, Zhang; Wuhan University. Renmin Hospital. Department of Organ Transplantation. Hubei. CN
  • Jilin, Zou; Wuhan University. Renmin Hospital. Department of Organ Transplantation. Hubei. CN
Acta cir. bras ; 34(4): e201900403, 2019. tab, graf
Article in En | LILACS | ID: biblio-1001087
Responsible library: BR1.1
ABSTRACT
Abstract

Purpose:

To investigate the long non-coding RNAs (lncRNAs) profile on renal ischemia reperfusion in a mouse model.

Methods:

Microarray analysis was used to study the expression of misregulated lncRNA in a mouse model of renal ischemia reperfusion(I/R) with long ischemia time. Quantitative real-time PCR (qPCR) was used to verify the expression of selected lncRNAs and mRNAs.The potential functions of the lncRNA was analyzed by bioinformatics tools and databases.

Results:

Kidney function was impaired in I/R group compared to the normal group. Analysis showed that a total of 2267 lncRNAs and 2341 messenger RNAs (mRNAs) were significantly expressed in I/R group (≥2.0-fold, p < 0.05).The qPCR result showed that lncRNAs and mRNAs expression were consistent with the microarray analysis. The co-expression network profile analysis based on five validated lncRNAs and 203 interacted mRNAs showed it existed a total of 208 nodes and 333 connections. The GO and KEEG pathway analysis results showed that multiple lncRNAs are involved the mechanism of I/R.

Conclusion:

Multiple lncRNAs are involved in the mechanism of I/R.These analysis results will help us to further understand the mechanism of I/R and promote the new methods targeted at lncRNA to improve I/R injury.
Subject(s)
Key words

Full text: 1 Collection: 01-internacional Database: LILACS Main subject: RNA, Messenger / Reperfusion Injury / RNA, Long Noncoding / Kidney Limits: Animals Language: En Journal: Acta cir. bras Journal subject: CIRURGIA GERAL / Procedimentos Cir£rgicos Operat¢rios Year: 2019 Document type: Article Affiliation country: China

Full text: 1 Collection: 01-internacional Database: LILACS Main subject: RNA, Messenger / Reperfusion Injury / RNA, Long Noncoding / Kidney Limits: Animals Language: En Journal: Acta cir. bras Journal subject: CIRURGIA GERAL / Procedimentos Cir£rgicos Operat¢rios Year: 2019 Document type: Article Affiliation country: China