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Regulation of ghrelin receptor by microbial and inflammatory signals in human osteoblasts
Nokhbehsaim, Marjan; Nogueira, Andressa Vilas Boas; Memmert, Svenja; Damanaki, Anna; Eick, Sigrun; Department of Diagnosis and SurgeryCirelli, Joni Augusto; Deschner, James.
Affiliation
  • Nokhbehsaim, Marjan; University of Bonn. Center of Dento-Maxillo-Facial Medicine. Section of Experimental Dento-Maxillo-Facial Medicine. Bonn. DE
  • Nogueira, Andressa Vilas Boas; University Medical Center. Johannes Gutenberg University. Department of Periodontology and Operative Dentistry. Mainz. DE
  • Memmert, Svenja; University of Bonn. Center of Dento-Maxillo-Facial Medicine. Department of Orthodontics. Bonn. DE
  • Damanaki, Anna; University Medical Center. Johannes Gutenberg University. Department of Periodontology and Operative Dentistry. Mainz. DE
  • Eick, Sigrun; University of Bern. Laboratory of Oral Microbiology. Department of Periodontology. Bern. CH
  • Department of Diagnosis and SurgeryCirelli, Joni Augusto; Universidade Estadual Paulista - UNESP. School of Dentistry of Araraquara. Department of Diagnosis and SurgeryCirelli, Joni Augusto. Araraquara. BR
  • Deschner, James; University Medical Center. Johannes Gutenberg University. Department of Periodontology and Operative Dentistry. Mainz. DE
Braz. oral res. (Online) ; 33: e025, 2019. graf
Article in En | LILACS | ID: biblio-1001603
Responsible library: BR1.1
ABSTRACT
Abstract Recently, it has been suggested that the anti-inflammatory hormone ghrelin (GHRL) and its receptor GHS-R may play a pivotal role in periodontal health and diseases. However, their exact regulation and effects in periodontitis are not known. The aim of this in-vitro study was to investigate the effect of microbial and inflammatory insults on the GHS-R1a expression in human osteoblast-like cells. MG-63 cells were exposed to interleukin (IL)-1β and Fusobacterium nucleatum in the presence and absence of GHRL for up to 2 d. Subsequently, gene expressions of GHS-R1a, inflammatory mediators and matrix metalloproteinase were analyzed by real-time PCR. GHS-R protein synthesis and NF-κB p65 nuclear translocation were assessed by immunocytochemistry and immunofluorescence microscopy, respectively. IL-1β and F. nucleatum caused a significant upregulation of GHS-R1a expression and an increase in GHS-R1a protein. Pre-incubation with a MEK1/2 inhibitor diminished the IL-1β-induced GHS-R1a upregulation. IL-1β and F. nucleatum also enhanced the expressions of cyclooxygenase 2, CC-chemokine ligand 2, IL-6, IL-8, and matrix metalloproteinase 1, but these stimulatory effects were counteracted by GHRL. By contrast, the stimulatory actions of IL-1β and F. nucleatum on the GHS-R1a expression were further enhanced by GHRL. Our study provides original evidence that IL-1β and F. nucleatum regulate the GHS-R/GHRL system in osteoblast-like cells. Furthermore, we demonstrate for the first time that the proinflammatory and proteolytic actions of IL-1β and F. nucleatum on osteoblast-like cells are inhibited by GHRL. Our study suggests that microbial and inflammatory insults upregulate GHS-R1a, which may represent a protective negative feedback mechanism in human bone.
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Full text: 1 Collection: 01-internacional Database: LILACS Main subject: Osteoblasts / Fusobacterium nucleatum / Interleukin-1beta / Receptors, Ghrelin Type of study: Evaluation_studies Limits: Humans Language: En Journal: Braz. oral res. (Online) Journal subject: ODONTOLOGIA Year: 2019 Document type: Article Affiliation country: Brazil / Germany / Switzerland Country of publication: Brazil

Full text: 1 Collection: 01-internacional Database: LILACS Main subject: Osteoblasts / Fusobacterium nucleatum / Interleukin-1beta / Receptors, Ghrelin Type of study: Evaluation_studies Limits: Humans Language: En Journal: Braz. oral res. (Online) Journal subject: ODONTOLOGIA Year: 2019 Document type: Article Affiliation country: Brazil / Germany / Switzerland Country of publication: Brazil