Investigation of biota associated and natural infection by sporulated bacteria in Culicidae larvae from São Paulo state, Brazil
Journal of Invertebrate Pathology
; 107(1): 11-15, Mai, 2011. ilus, tab
Article
in En
| SES-SP, SESSP-SUCENPROD, SES-SP
| ID: biblio-1064273
Responsible library:
BR93.2
Localization: BR93.2
ABSTRACT
The aim of this study was to investigate the naturally occurring bacteria from Culicidae larvae found in São Paulo state between 2006 and 2008 from the Tiete Ecological Park in the municipality of Caraguatatuba.Bacterial strains were obtained after surface sterilization of larvae followed by thermal treatmentand incubation in bacteriological media under laboratory conditions. Identification was determined using cytomorphology, biochemical and physiological tests. Strains were characterized by qualitative evaluationof biological activity against Culicidae larvae, comparing protein profiles obtained by electrophoresisof crystal protoxins, electrophoresis of Lysinibacillus sphaericus isoenzymes, detection of the L2 fraction of Hemolytic BL enterotoxin and amplification of DNA using Multiplex-PCR to detect HBL, NHE, CytK, BceT and EntFM enterotoxins. The results obtained in this study demonstrated that the endosymbiotic bacterial diversity belonging to Bacillus and related genera associated with Culicidae larvae is limited to a number of species that does not vary throughout the seasons...
Key words
Full text:
1
Collection:
06-national
/
BR
Database:
SES-SP
/
SESSP-SUCENPROD
Main subject:
Bacillus
/
Bacillus cereus
/
Bacillus thuringiensis
/
Biota
/
Culicidae
Type of study:
Qualitative_research
/
Risk_factors_studies
Limits:
Animals
Country/Region as subject:
America do sul
/
Brasil
Language:
En
Journal:
Journal of Invertebrate Pathology
Year:
2011
Document type:
Article