Long non-coding RNA MALAT1 regulates cell proliferation and apoptosis via miR-135b-5p/GPNMB axis in Parkinson's disease cell model
Biol. Res
; 54: 10-10, 2021. graf, ilus
Article
in En
| LILACS
| ID: biblio-1505803
Responsible library:
CL1.1
ABSTRACT
BACKGROUNDS Parkinson's disease (PD) is a common age-related neurodegenerative disorder worldwide. This research aimed to investigate the effects and mechanism underlying long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) in PD. METHODS:
SK-N-SH and SK-N-BE cells were treated with MPP+ to establish the MPP+-stimulated cell model of PD, and MALAT1 expression was determined. Then, the effects of MALAT1 depletion on cell proliferation and apoptosis were determined in the MPP+-stimulated cell model of PD. Besides, the correlations between microRNA-135b-5p (miR-135b-5p) and MALAT1 or glycoprotein nonmetastatic melanoma protein B (GPNMB) in MPP+-stimulated cell model of PD were explored.RESULTS:
MALAT1 was increasingly expressed and downregulation of MALAT1 promoted cell proliferation while inhibited apoptosis in MPP+-stimulated cells. Besides, miR-135b-5p was a target of MALAT1 and directly targeted to GPNMB. Further investigation indicated that suppression of MALAT1 regulated cell proliferation and apoptosis by miR-135b-5p/GPNMB axis.CONCLUSION:
Our findings reveal that MALAT1/miR-135b-5p/GPNMB axis regulated cell proliferation and apoptosis in MPP+-stimulated cell model of PD, providing a potential biomarker and therapeutic target for PD.Key words
Full text:
1
Collection:
01-internacional
Database:
LILACS
Main subject:
Parkinson Disease
/
Membrane Glycoproteins
/
Apoptosis
/
MicroRNAs
/
Cell Proliferation
/
RNA, Long Noncoding
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Biol. Res
Journal subject:
BIOLOGIA
Year:
2021
Document type:
Article
Affiliation country:
China
Country of publication:
Chile