Salivary Metabolites in Patients with Mucopolysaccharidosis
Pesqui. bras. odontopediatria clín. integr
; 24: e220151, 2024. graf
Article
in En
| LILACS, BBO
| ID: biblio-1535004
Responsible library:
BR1264.1
ABSTRACT
ABSTRACT Objective:
To identify the salivary metabolites profile of Mucopolysaccharidosis (MPS) types I, II, IV, and VI patients. Material andMethods:
The participants were asked to refrain from eating and drinking for one hour before sampling, performed between 730 and 900 a.m. Samples were centrifuged at 10.000 × g for 60 min at 4°C, and the supernatants (500µl) were stored at −80°C until NMR analysis. The salivary proton nuclear magnetic resonance (1H-NMR) spectra were acquired in a 500 MHz spectrometer, and TOCSY experiments were used to confirm and assign metabolites. Data were analyzed descriptively.Results:
Differences in salivary metabolites were found among MPS types and the control, such as lactate, propionate, alanine, and N-acetyl sugar. Understanding these metabolite changes may contribute to precision medicine and early detection of mucopolysaccharidosis and its monitoring.Conclusion:
The composition of low molecular weight salivary metabolites of mucopolysaccharidosis subjects may present specific features compared to healthy controls.Key words
Full text:
1
Collection:
01-internacional
Database:
BBO
/
LILACS
Main subject:
Saliva
/
Magnetic Resonance Spectroscopy
/
Mucopolysaccharidoses
/
Metabolomics
/
Proton Magnetic Resonance Spectroscopy
Limits:
Female
/
Humans
/
Male
Language:
En
Journal:
Pesqui. bras. odontopediatria clín. integr
Journal subject:
ODONTOLOGIA
Year:
2024
Document type:
Article
Affiliation country:
Brazil