Metabolic and ultrastructural renal changes in adult Wistar rats fed by a cafeteria diet
Acta cir. bras
; Acta cir. bras;39: e392224, 2024. tab, ilus
Article
in En
| LILACS, VETINDEX
| ID: biblio-1556673
Responsible library:
BR68.1
Localization: BR68.1
ABSTRACT
Purpose:
To evaluate, by quantitative and qualitative methods, the glomerular ultrastructure in Wistar rats fed a cafeteria diet.Methods:
Male Wistar rats were divided into two groups at 21 days of age control (C, n = 10) and cafeteria diet (CAF, n = 8). The animals were followed up until 5 months of age, followed by euthanasia. The blood, kidneys, and fat depositsepididymal, retroperitoneal, and subcutaneouswere extracted and analyzed. Data were analyzed by Student's t test, and p < 0.05 was considered statistically significant.Results:
The cafeteria diet promoted glucose intolerance, hyperglycemia (p < 0.0001), and deposition of retroperitoneal fat (p < 0.005). Scanning electron microscopy revealed that the length of the foot process was similar in both groups. The quantitative analyses by transmission electron microscopy revealed that the cafeteria diet reduced the thickness of the glomerular basement membrane (p < 0.05).Conclusions:
The intake of lipids and simple carbohydrates were found to be associated with alteration in the glomerular ultrastructure. However, more studies are needed to evaluate not only the effects of high-protein and high-fat diets on components of the glomerular filtration barrier, but also renal physiology.Key words
Full text:
1
Collection:
01-internacional
Database:
LILACS
/
VETINDEX
Main subject:
Microscopy, Electron, Scanning
/
Rats, Wistar
/
Diet
/
Kidney
Limits:
Animals
Language:
En
Journal:
Acta cir. bras
Journal subject:
Cirurgia Geral
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2024
Document type:
Article
Affiliation country:
Brazil
Country of publication:
Brazil