Removal and killing of multispecies endodontic biofilms by N-acetylcysteine
Braz. j. microbiol
; 49(1): 184-188, Jan.-Mar. 2018. tab, graf
Article
in En
| LILACS
| ID: biblio-889206
Responsible library:
BR1.1
ABSTRACT
ABSTRACT Removal of bacterial biofilm from the root canal system is essential for the management of endodontic disease. Here we evaluated the antibacterial effect of N-acetylcysteine (NAC), a potent antioxidant and mucolytic agent, against mature multispecies endodontic biofilms consisting of Actinomyces naeslundii, Lactobacillus salivarius, Streptococcus mutans and Enterococcus faecalis on sterile human dentin blocks. The biofilms were exposed to NAC (25, 50 and 100 mg/mL), saturated calcium hydroxide or 2% chlorhexidine solution for 7 days, then examined by scanning electron microscopy. The biofilm viability was measured by viable cell counts and ATP-bioluminescence assay. NAC showed greater efficacy in biofilm cell removal and killing than the other root canal medicaments. Furthermore, 100 mg/mL NAC disrupted the mature multispecies endodontic biofilms completely. These results demonstrate the potential use of NAC in root canal treatment.
Key words
Full text:
1
Collection:
01-internacional
Database:
LILACS
Main subject:
Acetylcysteine
/
Streptococcus mutans
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Actinomyces
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Enterococcus faecalis
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Biofilms
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Dental Pulp Diseases
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Ligilactobacillus salivarius
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Anti-Bacterial Agents
Limits:
Humans
Language:
En
Journal:
Braz. j. microbiol
Journal subject:
MICROBIOLOGIA
Year:
2018
Document type:
Article
/
Project document
Affiliation country:
South Korea