Exopolysaccharide production from Bacillus velezensis KY471306 using statistical experimental design
Braz. j. microbiol
; 49(3): 452-462, July-Sept. 2018. tab, graf
Article
in En
| LILACS
| ID: biblio-951792
Responsible library:
BR1.1
ABSTRACT
Abstract Exopolysaccharide (EPS) biopolymers produced by microorganisms play a crucial role in the environment such as health and bio-nanotechnology sectors, gelling agents in food and cosmetic industries in addition to bio-flocculants in the environmental sector as they are degradable, nontoxic. This study focuses on the improvement of EPS production through manipulation of different culture and environmental conditions using response surface methodology (RSM). Plackett-Burman design indicated that; molasses, yeast extract and incubation temperature are the most effective parameters. Box-Behnken RSM indicated that; the optimum concentration for each parameter was 12% (w/v) for molasses, 6 g/L yeast extract and 30 °C for incubation temperature. The most potent bacterial isolate was identified as Bacillus velezensis KY498625. After production, EPS was extracted, purified using DEAE-cellulose, identified using Fourier transform infrared (FTIR), gel permeation chromatography (GPC) and gas chromatography-mass spectroscopy (GC-MS). The result indicated that; it has molecular weight 1.14 × 105 D consisting of glucose, mannose and galactose.
Key words
Full text:
1
Collection:
01-internacional
Database:
LILACS
Main subject:
Polysaccharides, Bacterial
/
Bacillus
Language:
En
Journal:
Braz. j. microbiol
Journal subject:
MICROBIOLOGIA
Year:
2018
Document type:
Article
Affiliation country:
Egypt