Antibacterial and Cytotoxic Potential of a Brazilian Red Propolis
Pesqui. bras. odontopediatria clín. integr
; 19(1): 4626, 01 Fevereiro 2019. tab, graf
Article
in En
| LILACS, BBO
| ID: biblio-998263
Responsible library:
BR1264.1
ABSTRACT
Objective:
To evaluate in vitro the effect of a red propolis ethanolic extract (RPE) in the prevention of growth of a cariogenic biofilm and its cytotoxic potential. Material andMethods:
Minimum inhibitory and bactericidal concentrations (MIC and MBC) of RPE against Streptococcus mutans and Lactobacillus casei were determined. The cytotoxic potential of 0.4% RPE in oral fibroblasts was observed after 1, 3 and 5 min of contact. Cellulose membrane disks (13 mm, N=12) were used for biofilm formation (24 h) of S. mutans and L. casei, which were treated (1 min) with 0.4% RPE or 0.12% Chlorhexidine (CHX). The control group of biofilm formation was not submitted to any treatment. Serial dilutions were then made to evaluate microbial viability. Descriptive data analysis and, for microbial viability, Mann Whitney test were performed (p≤0.05).Results:
RPE showed similar MIC and MBC (4.46 mg/mL) against S. mutans and, for L. casei, they were 8.92 mg/mL (MIC) and 17.85 mg/mL (MBC). CHX presented MIC and MBC <0.00002 mg/mL for S. mutans and 0.00047 mg/mL for L. casei. After 1, 3 and 5 min, the RPE exhibited, respectively, 69.38%, 43.91% and 40.36% of viable cells. The RPE (6.55) and CHX (6.87) presented similar efficacy to reduce the total number of viable bacteria (p>0.05). Regarding the total number of viable bacteria (Log10 CFU/mL), the RPE (6.55) and CHX (6.87) presented similar efficacy (p>0.05).Conclusion:
Red propolis extract showed antibacterial activity against the tested strains, exhibited acceptable cytotoxicity and reduced the colonization of S. mutans and L. casei in a biofilm membrane model.Key words
Full text:
1
Collection:
01-internacional
Database:
BBO
/
LILACS
Main subject:
Propolis
/
In Vitro Techniques
/
Plant Extracts
/
Biofilms
/
Anti-Bacterial Agents
Type of study:
Evaluation_studies
Country/Region as subject:
America do sul
/
Brasil
Language:
En
Journal:
Pesqui. bras. odontopediatria clín. integr
Journal subject:
ODONTOLOGIA
Year:
2019
Document type:
Article
Country of publication:
Brazil