Enzymatic method for branched chain alpha-ketoacid determination: application to rapid analysis of urine and plasma samples from maple syrup urine disease patients.

Burgos, C; Civallero, G E; de Kremer, R D; Gerez de Burgos, N M; Blanco, A

Burgos, C; Civallero, G E; de Kremer, R D; Gerez de Burgos, N M; Blanco, A.

Affiliation

Blanco, A; Catedra de Química Biológica, Facultad de Ciencias Médicas, Córdoba, Argentina. cburgose biomed.fcm.unc.edu.ar.

Acta Physiol. Pharmacol. Ther Latinoam ; 49(2): 109-17, 1999.

Article in En | BINACIS | ID: bin-40130

Responsible library: AR1.1

ABSTRACT

ABSTRACT

A new method for the determination of branched-chain alpha-ketoacid concentration using lactate dehydrogenase (E C 1.1.1.27) isozyme C4 (LDH C4) from mouse testes is proposed. The assay is performed on urine and plasma without previous treatment. Alpha-ketoglutarate and pyruvate are determined on the same sample using glutamate dehydrogenase (GDH, EC 1.4.1.2) and lactate dehydrogenase isozyme A4 (LDH5) respectively and subtracted from the total alpha-ketoacid concentration obtained with LDH C4. This value corresponds to the branched chain alpha-ketoacid. Results were linear within the concentration range 8 to 170 mumoles/L. Detection limit was 8 mumoles/L. Analytical recovery was higher than 91

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Collection: 06-national / AR Database: BINACIS Language: En Journal: Acta Physiol. Pharmacol. Ther Latinoam Year: 1999 Document type: Article Country of publication: Argentina

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