Role of nitric oxide and signaling pathways modulating the stimulatory effect of snake venom secretory PLA2S on non-opsonized zymosan phagocytosis by macrophages
Toxicon, v. 243, 107716, mai. 2024
Article
in En
| SES-SP, SESSP-IBPROD, SES-SP
| ID: bud-5322
Responsible library:
BR78.1
ABSTRACT
The phagocytic activity of macrophages activated with MT-II, a Lys-49 PLA2 homolog, and MT-III, an Asp-49 PLA2, from Bothrops asper snake venom, was investigated in this study using a pharmacological approach. Stimulating thioglycollate-elicited macrophages with both venom components enhanced their ability to phagocytose non-opsonized zymosan particles. MT-II and MT–III–induced phagocytosis was drastically inhibited by pretreating cells with L-NAME, aminoguanidine or L-NIL, cNOS or iNOS inhibitors, or with ODQ (sGC inhibitor) or Rp-cGMPS (PKG inhibitor). These results indicate that the NO/sGC/GMP/PKG pathway plays an essential role in the β-glucan-mediated phagocytosis induced in macrophages by these venom-secretory PLA2s.
Full text:
1
Collection:
06-national
/
BR
Database:
SES-SP
/
SESSP-IBPROD
Type of study:
Screening_studies
Language:
En
Journal:
Toxicon
Year:
2024
Document type:
Article