Changes in the inmunne cell p opulation and cell proliferation in peripheral blood after gemcitabine-based chemotherapy for pancreatic cancer
Clin. transl. oncol. (Print)
; 16(3): 330-335, mar. 2014.
Article
in En
| IBECS
| ID: ibc-127742
Responsible library:
ES1.1
Localization: BNCS
ABSTRACT
PURPOSE: Regulatory T cells (Tregs) play a role in the immunosuppressive state in pancreatic cancer patients. We aimed to evaluate the changes of immune cells population including Tregs caused by gemcitabine (GEM)-based chemotherapy. METHODS: Fifty-three patients with pancreatic cancer were enrolled in this study, of which 32 received GEM- based chemotherapy. Blood samples were collected before and at least 2 weeks after the last dose of chemotherapy. The peripheral blood mononuclear cells (PBMCs) were subjected to flow cytometry analysis after labeling with anti-CD4, anti-CD25, and anti-Foxp3 antibodies. Other lymphocytes and NK cell markers were also measured. The proliferative capacity of PBMCs stimulated with anti-CD3 was analyzed using H(3) thymidine. RESULTS: The percentage and number of Tregs were significantly decreased after chemotherapy (p = 0.032, p = 0.003, respectively). The other immune cells and the proliferative capacity did not change. CONCLUSION: This study showed that GEM-based chemotherapy produced an immunomodulatory effect via the depletion of Tregs (AU)
RESUMEN
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Collection:
06-national
/
ES
Database:
IBECS
Main subject:
Pancreatic Neoplasms
/
T-Lymphocytes, Regulatory
/
Cell Proliferation
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
En
Journal:
Clin. transl. oncol. (Print)
Year:
2014
Document type:
Article