The B2 - adrenoreceptor gene promoter polymorphisms may modulate B2 - agonist and glucocorticoid-induced IgE synthesis
Allergol. immunopatol
; 42(6): 586-593, nov.-dic. 2014. tab, graf
Article
in English
| IBECS
| ID: ibc-130150
Responsible library:
ES1.1
Localization: BNCS
ABSTRACT
BACKGROUND:
β2-adrenoreceptor (β2-AR) agonists and glucocorticoids (GCS) were shown to induce IgE synthesis in human PBMCs. Serum total IgE levels are associated with single nucleotide polymorphisms (SNPs) of the β2-AR gene. We aimed to assess the association of the effect of fenoterol (β2-AR agonist) on IL-4-driven and budesonide-induced IgE synthesis with genetic variants of β2-AR.METHODS:
The study included 25 individuals 13 with allergic asthma and/or allergic rhinitis and 12 healthy volunteers. PBMCs were cultured with IL-4, fenoterol and/or budesonide, and IgE concentrations in supernatants were assessed. Five SNPs in positions −47, −20, 46, 79 and 252 of β2-AR were determined by direct DNA sequencing.RESULTS:
In −47 T/T and −20 T/T patients, incubation with fenoterol resulted in decreased IgE production, whereas in −47 C/T and −47 C/C as well as in −20 C/T and −20 C/C individuals, it was enhanced. In contrast to fenoterol, budesonide-induced IgE synthesis was significantly increased in −47 T/T and −20 T/T patients as compared to −47 C/T, −47 C/C, −20 C/T and −47 C/C individuals. Polymorphisms in positions 46, 79 and 252 were not associated with fenoterol- or budesonide-modulated IgE synthesis. No differences in the distribution of IgE synthesis was seen between atopic and non-atopic individuals carrying the same alleles.CONCLUSIONS:
The differential effect of β2-agonists and GCS on IgE synthesis may be associated with genetic variants of promoter region of the β2-AR geneRESUMEN
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Collection:
National databases
/
Spain
Database:
IBECS
Main subject:
Asthma
/
Immunoglobulin E
/
Adrenergic beta-Antagonists
Limits:
Humans
Language:
English
Journal:
Allergol. immunopatol
Year:
2014
Document type:
Article
Institution/Affiliation country:
Medical University of Lodz/Poland