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MicroRNA-33b inhibits tumor cell growth and is associated with prognosis in colorectal cancer patients
Liao, W; Gu, C; Huang, A; Yao, J; Sun, R.
Affiliation
  • Liao, W; Fudan University, No. 1508. Jinshan Hospital. Department of General Surgery. Shanghai. Republic of China
  • Gu, C; Fudan University, No. 1508. Jinshan Hospital. Department of General Surgery. Shanghai. Republic of China
  • Huang, A; Fudan University, No. 1508. Jinshan Hospital. Department of General Surgery. Shanghai. Republic of China
  • Yao, J; Fudan University, No. 1508. Jinshan Hospital. Department of General Surgery. Shanghai. Republic of China
  • Sun, R; Fudan University, No. 1508. Jinshan Hospital. Department of General Surgery. Shanghai. Republic of China
Clin. transl. oncol. (Print) ; 18(5): 449-456, mayo 2016. tab, graf
Article in En | IBECS | ID: ibc-151177
Responsible library: ES1.1
Localization: BNCS
ABSTRACT
Purpose: To explore the role of miR-33b in colorectal cancer (CRC) and the correlation between its expression and prognosis. Methods: The expressions of miR-33b between CRC tissues and normal tissues were measured by real-time PCR. The effects of miR-33b on cell proliferation and cell cycle progression were detected by MTT assay, colony formation assay and flow cytometry. The potential regulations of miR-33b on multiple genes expression were verified by Western blot. Furthermore, the association of miR-33b with CRC clinicopathologic features and prognosis was analyzed by Chi-squared test and Kaplan-Meier tests. Results: MiR-33b was downregulated in CRC compared with normal colorectal samples and miR-33b inhibited tumor cell growth and induced cell cycle arrest. Western blot assays and correlation analysis showed that miR-33b could regulate multiple growth-related genes. Moreover, the expression of miR-33b was associated with TNM stage and tumor size, and CRC patients with high miR-33b expression had a better prognosis. Conclusion: Our data suggest that miR-33b functions as a tumor suppressor gene in CRC through regulating cell proliferation and cell cycle (AU)
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Collection: 06-national / ES Database: IBECS Main subject: Prognosis / RNA / Colorectal Neoplasms / Cell Cycle / Blotting, Western / Flow Cytometry Type of study: Evaluation_studies / Prognostic_studies / Risk_factors_studies Limits: Female / Humans / Male Language: En Journal: Clin. transl. oncol. (Print) Year: 2016 Document type: Article
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Add to My VHL
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Collection: 06-national / ES Database: IBECS Main subject: Prognosis / RNA / Colorectal Neoplasms / Cell Cycle / Blotting, Western / Flow Cytometry Type of study: Evaluation_studies / Prognostic_studies / Risk_factors_studies Limits: Female / Humans / Male Language: En Journal: Clin. transl. oncol. (Print) Year: 2016 Document type: Article