Disintegrin and metalloproteinases (ADAMs) expression in gastroesophageal reflux disease and in esophageal adenocarcinoma
Clin. transl. oncol. (Print)
; 19(1): 58-66, ene. 2017. tab, graf, ilus
Article
in En
| IBECS
| ID: ibc-159119
Responsible library:
ES1.1
Localization: BNCS
ABSTRACT
Background. Clinically useful marker molecules for the progression of gastroesophageal reflux disease and Barretts esophagus (BE) to esophageal adenocarcinoma (EAC) are lacking. Many adenocarcinomas and inflammatory conditions exhibit increased expression of ADAMs, a disintegrin and metalloproteinases. Methods. We assessed the expression of five ADAMs (9, 10, 12, 17, 19) in three esophageal cell lines (Het-1A, OE19, OE33) by RT-PCR and Western blotting, and in human samples of normal esophagus, esophagitis, BE, Barretts dysplasia, and EAC by RT-PCR, and in selected samples by immunohistochemistry. Results. EAC patients showed increased mRNA expression of ADAMs 9, 12, 17 and 19, as compared to controls. At immunohistochemistry, ADAM9 and ADAM10 proteins were increased in EAC. Patient samples also showed increased mRNA expression of ADAM12 in esophagitis, of ADAM9 in BE, and of ADAMs 9, 12 and 19 in Barretts dysplasia, as compared to controls. Two EAC cell lines showed increased ADAM9 mRNA. Conclusions. ADAM9 expression is increased in EAC. Its predecessors show increased ADAM9 mRNA expression. The importance of the alterations in ADAM expression for the development of EAC, and their use as marker molecules, warrant further studies (AU)
RESUMEN
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Collection:
06-national
/
ES
Database:
IBECS
Main subject:
Barrett Esophagus
/
Adenocarcinoma
/
Clinical Protocols
/
Tissue Inhibitor of Metalloproteinases
/
Metalloproteases
/
Duodenogastric Reflux
Type of study:
Guideline
Limits:
Female
/
Humans
/
Male
Language:
En
Journal:
Clin. transl. oncol. (Print)
Year:
2017
Document type:
Article