Kaempferol inhibits the production of ROS to modulate OPN-alfavbeta3 integrin pathway in HUVECs
J. physiol. biochem
; 72(2): 303-313, jun. 2016. ilus, graf
Article
in En
| IBECS
| ID: ibc-168274
Responsible library:
ES1.1
Localization: BNCS
ABSTRACT
In the present study, we tested the hypothesis that aldosterone regulates osteopontin (OPN)-related signaling pathways to promote nuclear factor κB (NF-κB) activation in primary human umbilical vein endothelial cells (HUVECs) and that kaempferol, a flavonoid compound, blocks those changes. Aldosterone induced productions of reactive oxygen species (ROS), OPN, interleukin-6 (IL-6), and tumor necrosis factor alpha (TNF-α) and expression of nicotinamide adenine dinucleotide phosphate-oxidase 4 (Nox4), NF-κB, OPN, alphavbeta3 (αvβ3) integrin, and inhibitor of NF-κB alpha phosphorylation (P-IκBα) in HUVEC. HUVECs were pretreated with kaempferol (0, 1, 3, or 10 μM) for 1 h and exposed to aldosterone (10−6 M) for 24 h. Kaempferol reduced ROS, OPN, NF-κB, IL-6, and TNF-α levels; Nox4, αvβ3 integrin; and P-IκBα expressions. The effect of aldosterone was also abrogated by spironolactone (10−6 M). In addition, vitamin C (20 mmol/L) reduced ROS production. Vitamin C and LM609 (10 μg/mL) treatment decreased expressions of OPN, αvβ3 integrin, and NF-κB (P < 0.05 or P < 0.01). The present results suggest that kaempferol may modulate OPN-αvβ3 integrin pathway to inhibit NF-κB activation in HUVECs (AU)
RESUMEN
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Collection:
06-national
/
ES
Database:
IBECS
Main subject:
Endothelium, Vascular
/
Signal Transduction
/
Down-Regulation
/
Anti-Inflammatory Agents, Non-Steroidal
/
Reactive Oxygen Species
/
Kaempferols
/
Antioxidants
Limits:
Humans
Language:
En
Journal:
J. physiol. biochem
Year:
2016
Document type:
Article