Fine particulate matter potentiates type 2 diabetes development in high-fat diet-treated mice: stress response and extracellular to intracellular HSP70 ratio analysis
J. physiol. biochem
; 72(4): 643-656, dic. 2016. ilus, graf
Article
in English
| IBECS
| ID: ibc-168372
Responsible library:
ES1.1
Localization: BNCS
ABSTRACT
Exposure to fine particulate matter (PM2.5) air pollution is a risk factor for type 2 diabetes (T2DM). We argue whether the potentiating effect of PM2.5 over the development of T2DM in high-fat diet (HFD)-fed mice would be related to modification in cell stress response, particularly in antioxidant defenses and 70-kDa heat shock proteins (HSP70) status. Male mice were fed standard chow or HFD for 12 weeks and then randomly exposed to daily nasotropic instillation of PM2.5 for additional 12 weeks under the same diet schedule, divided into four groups (n = 14-15 each) Control, PM2.5, HFD, and HFD + PM2.5 were evaluated biometric and metabolic profiles of mice, and cellular stress response (antioxidant defense and HSP70 status) of metabolic tissues. Extracellular to intracellular HSP70 ratio ([eHSP72]/[iHSP70]), viz. H-index, was then calculated. HFD + PM2.5 mice presented a positive correlation between adiposity, increased body weight and glucose intolerance, and increased glucose and triacylglycerol plasma levels. Pancreas exhibited lower iHSP70 expression, accompanied by 3.7-fold increase in the plasma to pancreas [eHSP72]/[iHSP70] ratio. Exposure to PM2.5 markedly potentiated metabolic dysfunction in HFD-treated mice and promoted relevant alteration in cell stress response assessed by [eHSP72]/[iHSP70], a relevant biomarker of chronic low-grade inflammatory state and T2DM risk (AU)
RESUMEN
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Collection:
National databases
/
Spain
Database:
IBECS
Main subject:
Glucose Intolerance
/
HSP70 Heat-Shock Proteins
/
Diabetes Mellitus, Type 2
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HSP72 Heat-Shock Proteins
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Particulate Matter
/
Obesity
Type of study:
Risk factors
Limits:
Animals
Language:
English
Journal:
J. physiol. biochem
Year:
2016
Document type:
Article
Institution/Affiliation country:
Federal University of Health Sciences of Porto Alegre (UFCSPA)/Brazil
/
Federal University of Rio Grande do Sul (UFRGS)/Brazil
/
Regional University of Northwestern/Brazil