Human parvovirus B 19 infection and hydrops fetalis in Rio de Janeiro, Brazil
Mem. Inst. Oswaldo Cruz
; 91(2): 147-51, Mar.-Apr. 1996. tab
Article
in En
| LILACS
| ID: lil-174368
Responsible library:
BR15.1
RESUMO
Formalin-fixed paraffin embedded lung and liver tissue from 23 cases of non immune hydrops fetalis and five control cases, in which hydrops were due to syphilis (3) and genetics causes (2), were examined for the presence of human parvovirus B19 by DNA hybridisation. Using in situ hybridisation with a biotynilated probe one positive case was detected. Using 32 P-labelled probes in a dot blot assay format, five further positives were obtained. These were all confirmed as positive by a nested polymerase chain reaction assay. Electron microscopy revealed virus in all these five positive cases. The six B19 DNA positive cases of hydrops fetalis were from 1974, 1980, 1987 and 1988, four of which occurred during the second half of the year, confirming the seasonality of the disease.
Full text:
1
Collection:
01-internacional
Database:
LILACS
Main subject:
Parvoviridae
/
Blood
Type of study:
Diagnostic_studies
Limits:
Adult
/
Humans
Country/Region as subject:
America do sul
/
Brasil
Language:
En
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
MEDICINA TROPICAL
/
PARASITOLOGIA
Year:
1996
Document type:
Article