Analysis of parity between protein-based electrophoretic methods for the characterization of oral Candida species
Mem. Inst. Oswaldo Cruz
; 95(6): 801-6, Nov.-Dec. 2000. tab
Article
in En
| LILACS
| ID: lil-273434
Responsible library:
BR1.1
ABSTRACT
Electrophoretic studies of multilocus-enzymes (MLEE) and whole-cell protein (SDS-PAGE) were carried out in order to evaluate the parity between different methods for the characterization of five Candida species commonly isolated from oral cavity of humans by numerical taxonomy methods. The obtained data revealed that sodium dodecyl sulfate polyacrylamide gel electrophoresis is more efficient in grouping strains in their respective species while MLEE has much limited resolution in organizing all strains in their respective species-specific clusters. MLEE technique must be regarded for surveys in which just one species of Candida is involved
Full text:
1
Collection:
01-internacional
Database:
LILACS
Main subject:
Candida
/
Proteins
/
Electrophoresis
/
Enzymes
/
Mouth
Limits:
Humans
Language:
En
Journal:
Mem Inst Oswaldo Cruz
/
Mem. Inst. Oswaldo Cruz
/
Mem. Inst. Oswaldo Cruz (Online)
/
Memórias do Instituto Oswaldo Cruz (Impresso)
/
Proceedings of the Instituto Oswaldo Cruz
Journal subject:
MEDICINA TROPICAL
/
PARASITOLOGIA
Year:
2000
Document type:
Article
Affiliation country:
Brazil
Country of publication:
Brazil