Leukotoxic activity of Actinobacillus Actinomycetemcomitans isolated from human and non-human primates PRIMATES
Braz. j. microbiol
; 32(3): 248-254, July-Sept., 2001. tab, graf
Article
in English
| LILACS
| ID: lil-316980
Responsible library:
BR32.1
RESUMO
Actinobacillus actinomycetemcomitans is a clinically relevant periodontopathogenic Gram-negative coccobacillus that produces a leukotoxin of the RTX cytolysin family. In this study, we evaluated the leukotoxic activity of A. actinomycetemcomitans strains isolated from human and marmosets by Trypan blue exclusion and by the chemiluminescence assays. Among eight A. actinomycetemcomitans human strains studied, two (P2.17 and P8.12) were classified as high leukotoxin producers and among eight marmoset strains, one (M22.11) showed high leukotoxin production, as determined by Trypan blue exclusion assay. The reference strains ATCC 29523 and FDC Y4 respectively behaved like moderate and low producers. The chemiluminescence assay was used to evaluate the leukotoxic activity of M22.11 and P2.17 strains submitted to different growth conditions. Leukotoxic activity was detected on cells at the logarithmic phase and was similar under anaerobic and microaerophilic growth conditions. It was greatly reduced when cells were grown at glucose concentrations lower or higher than 0.75per cent (0.25per cent and 1.5per cent) in thioglycolate medium. Leukotoxin production mainly by the M22.11 strain was low in BHI broth, whereas production in TSB medium showed a similar level as in thioglycolate broth medium. Sodium bicarbonate at 10 mM did not affect leukotoxin production.
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
Actinobacillus Infections
/
Chemotaxis, Leukocyte
/
Actinobacillus
Limits:
Animals
/
Humans
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2001
Document type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Estadual do Piauí/BR
/
Universidade Federal de Minas Gerais/BR