Microtubule cytoskeleton distribution using fluorescent taxoid in tetratrichomonas didelphidis
Parasitol. latinoam
; 58(3/4): 101-105, jul. 2003. ilus
Article
in En
| LILACS
| ID: lil-383486
Responsible library:
CL1.1
RESUMO
Tetratrichomonas didelphidis is a flagellated protozoan found in the intestine of opossums. The specimens were stained by the Giemsa method and by FLUTAX-2, an active fluorescent derivative of Taxol which binds to the ab-tubulin polimerized of microtubules of cells. Giemsa stain revealed the morphological features of trichomonads such as four anterior flagella, undulating membrane, axostyle and posterior flagellum. An intense fluorescence was observed in living trophozoites of T. didelphidis and Trichomonas vaginalis (used as control), incubated with FLUTAX-2. An analysis of the composition of the cytoskeleton of T. didelphidis will contribute to understanding the cellular morphology of the parasites. Key words Tetratrichomonas didelphidis, microtubule cytoskeleton, fluorescent taxoid.
Full text:
1
Collection:
01-internacional
Database:
LILACS
Main subject:
Trichomonas
/
Cytoskeleton
/
In Vitro Techniques
/
Fluorescent Dyes
/
Microtubules
Limits:
Animals
Language:
En
Journal:
Parasitol. latinoam
Journal subject:
PARASITOLOGIA
Year:
2003
Document type:
Article
Affiliation country:
Brazil