Efectos de la malnutricion proteico-calorica materna sobre la concentracion del ARN mensajero de la proteina A del surfactante en pulmones de ratas fetales / Effects of maternal protein-calorie malnutrition on the concentration of protein A messenger RNA in surfactant of fetal rat lungs
Rev. méd. Panamá
; 24(1): 26-33, Jan.-May 1999.
Article
in Es
| LILACS
| ID: lil-409807
Responsible library:
BR1.1
ABSTRACT
The pulmonary surfactant is a lipoproteic complex that serves to lower surface tension at the air-liquid interface in the pulmonary alveoli. Approximately 2 to 4% of the pulmonary surfactant is constituted by the protein A (SP-A). The objective of the study was to determine the effects that maternal protein calorie malnutrition has on the fetal pulmonary growth and the production of SP-A messenger RNA in fetal rats. MATERIALS AND METHODS:
Pregnant Sprague Dawley rats were divided into two groups, which received a diet with either 8% or 21% of proteins from gestational day 1 until the day 20. In this last day 11 fetuses were extracted by caesarean section and their lungs were removed to quantify the mRNA of the SP-A. First the mRNA was boosted using the technique of reverse transcriptase and polimerase chain reaction (RT-PCR) and then its concentration was determined by means of fluorodensitometry.RESULTS:
There was a reduction in body weight and in wet lung weights of malnourished fetuses in comparison with the normal fetuses (5.03 +/- 0.20 g vs. 4.32 +/- 0.32 g, p < 0.05 and 79.0 +/- 3.8 mg vs. 146.0 +/- 3.4 mg, p < 0.05, respectively). The densitometric analysis of the SP-A mRNA concentration demonstrated a reduction of 32% in the malnourished fetuses (0.52 +/- 0.11 vs. 0.77 +/- 0.07, p < 0.05) compared with the normal fetuses.CONCLUSIONS:
The maternal protein calorie malnutrition affected the pulmonary development and the synthesis of the SP-A mRNA. These data suggest that a defect occurrs at pre-transcriptional level that results in a diminution of the concentration of mRNA of SP-A in the neumocytes type II
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Collection:
01-internacional
Database:
LILACS
Main subject:
Pregnancy Complications
/
Pulmonary Surfactants
/
RNA, Messenger
/
Protein-Energy Malnutrition
/
Pulmonary Surfactant-Associated Protein A
/
Lung
Limits:
Animals
/
Pregnancy
Language:
Es
Journal:
Rev. méd. Panamá
Journal subject:
MEDICINA
Year:
1999
Document type:
Article
Affiliation country:
Panama