Application of biochemical and polymerase chain reaction assays for identification of Campylobacter isolates from non-human primates
Mem. Inst. Oswaldo Cruz
; 101(5): 499-501, Aug. 2006. ilus, tab
Article
in English
| LILACS
| ID: lil-437033
Responsible library:
BR1.1
ABSTRACT
A multiplex polymerase chain reaction (PCR) assay was performed on 167 thermophilic campylobacters isolated from non-human primates. Samples were first identified by phenotypic methods resulting in 64 Campylobacter jejuni and 103 C. coli strains. Four strains identified biochemically as C. coli, were then determined to be C. jejuni by PCR. Comparison of methodologies showed that the main discrepancies were attributed to the hippurate hydrolysis test and sensitivity to cephalothin and nalidixic acid. Analysis of data showed that the application of phenotypic methods should be supplemented by a molecular method to offer a more reliable Campylobacter identification.
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
Campylobacter
/
DNA, Bacterial
/
Polymerase Chain Reaction
/
Bacterial Typing Techniques
Type of study:
Diagnostic study
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2006
Document type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Fundação Oswaldo Cruz/BR
/
Universidade Federal do Rio de Janeiro/BR