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Noninvasive genetic sampling of endangered muriqui (Primates, Atelidae): efficiency of fecal DNA extraction
Chaves, Paulo B; Paes, Marcela F; Mendes, Sérgio L; Strier, Karen B; Louro, Iúri D; Fagundes, Valéria.
Affiliation
  • Chaves, Paulo B; Universidade Federal do Espírito Santo. Departamento de Ciências Biológicas. Laboratório de Genética Animal. Vitória. BR
  • Paes, Marcela F; Universidade Federal do Espírito Santo. Departamento de Ciências Biológicas. Laboratório de Genética Animal. Vitória. BR
  • Mendes, Sérgio L; Universidade Federal do Espírito Santo. Departamento de Ciências Biológicas. Laboratório de Biologia da Conservação de Vertebrados. Vitória. BR
  • Strier, Karen B; University of Wisconsin-Madison. Department of Anthropology. Madison. US
  • Louro, Iúri D; Universidade Federal do Espírito Santo. Departamento de Ciências Biológicas. Núcleo de Genética Humana e Molecular. Vitória. BR
  • Fagundes, Valéria; Universidade Federal do Espírito Santo. Departamento de Ciências Biológicas. Laboratório de Genética Animal. Vitória. BR
Genet. mol. biol ; 29(4): 750-754, 2006. ilus
Article in En | LILACS | ID: lil-450501
Responsible library: BR26.1
ABSTRACT
The muriqui (Brachyteles) is one of the most endangered primates in the world, however little is known about the viability of the remaining populations. We evaluated the technique of extracting DNA from wild muriqui feces for PCR applications. In order to determine the effect of the DNA in subsequent amplifications, we analyzed five different extracts. The importance of the recommended BSA and the HotStarTaq DNA polymerase was tested. The minimal conditions to successfully amplify highly degraded fecal DNA were determined, showing that the recommended reagents are not required. We envision that this method may be useful in further conservation management studies.
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Full text: 1 Collection: 01-internacional Database: LILACS Main subject: Primates / DNA / Feces Limits: Animals / Humans Language: En Journal: Genet. mol. biol Journal subject: GENETICA Year: 2006 Document type: Article Affiliation country: Brazil / United States
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Full text: 1 Collection: 01-internacional Database: LILACS Main subject: Primates / DNA / Feces Limits: Animals / Humans Language: En Journal: Genet. mol. biol Journal subject: GENETICA Year: 2006 Document type: Article Affiliation country: Brazil / United States