First isolation of microorganisms from the gut diverticulum of Aedes aegypti (Diptera: Culicidae): new perspectives for an insect-bacteria association
Mem. Inst. Oswaldo Cruz
; 102(8): 919-924, Dec. 2007. ilus
Article
in En
| LILACS
| ID: lil-471856
Responsible library:
BR1.1
ABSTRACT
We show for the first time that the ventral diverticulum of the mosquito gut (impermeable sugar storage organ) harbors microorganisms. The gut diverticulum from newly emerged and non-fed Aedes aegypti was dissected under aseptic conditions, homogenized and plated on BHI medium. Microbial isolates were identified by sequencing of 16S rDNA for bacteria and 28S rDNA for yeast. A direct DNA extraction from Ae. aegypti gut diverticulum was also performed. The bacterial isolates were Bacillus sp., Bacillus subtilis and Serratia sp. The latter was the predominant bacteria found in our isolations. The yeast species identified was Pichia caribbica.
Key words
Full text:
1
Collection:
01-internacional
Database:
LILACS
Main subject:
Pichia
/
Serratia
/
Bacillus
/
Aedes
/
Gastrointestinal Tract
Type of study:
Risk_factors_studies
Limits:
Animals
Language:
En
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
MEDICINA TROPICAL
/
PARASITOLOGIA
Year:
2007
Document type:
Article
Affiliation country:
Brazil