Detection of brucella ovis in ovine from Paraíba state, in the northeast region of Brazil
Braz. j. microbiol
; 41(2): 365-367, Apr.-June 2010. ilus
Article
in En
| LILACS
| ID: lil-545343
Responsible library:
BR32.1
ABSTRACT
To determine the presence of Brucella ovis in ovine from Paraíba State, in the Northeast region of Brazil, 80 animals slaughtered in the public slaughterhouse of Patos city were used. Before slaughter, blood samples were collected by jugular venopuncture from each animal, and after slaughter, testicles, epidydimus and uterus were aseptically collected. For the serological diagnosis of B. ovis and B. abortus infections, the agar gel immunodiffusion (AGID) and Rose Bengal (RBT) tests were carried out, respectively. In addition, microbiological culture and polymerase chain reaction (PCR) were performed on testicle, epidydimus and uterus samples. Six animals (7.5 percent) tested positive for the presence of B. ovis antibodies and all animals tested negative for the presence of B. abortus antibodies. One AGID-positive animal tested positive at uterine swab culture. PCR was able to amplify DNA of Brucella spp. from the pool of testicle, epidydimus and uterus samples from AGID-positive animals. This is the first report of isolation and detection of B. ovis DNA by PCR in ovine from the Northeast region of Brazil.
Key words
Full text:
1
Collection:
01-internacional
Database:
LILACS
Main subject:
In Vitro Techniques
/
Brucellosis, Bovine
/
Polymerase Chain Reaction
/
Brucella ovis
Type of study:
Diagnostic_studies
Limits:
Animals
Country/Region as subject:
America do sul
/
Brasil
Language:
En
Journal:
Braz. j. microbiol
Journal subject:
MICROBIOLOGIA
Year:
2010
Document type:
Article
Affiliation country:
Brazil
Country of publication:
Brazil