Enhancing T3 and cAMP responsive gene participation in the thermogenic regulation of fuel oxidation pathways / Ampliando a participação dos genes responsivos a T3 e cAMP na regulação da termogênese gerada pelas vias de oxidação biológica
Arq. bras. endocrinol. metab
; 54(4): 381-389, jun. 2010. ilus, tab
Article
in En
| LILACS
| ID: lil-550708
Responsible library:
BR1.1
ABSTRACT
OBJECTIVE:
We sought to identify glycolysis, glycogenolysis, lipolysis, Krebs cycle, respiratory chain, and oxidative phosphorylation enzymes simultaneously regulated by T3 and cAMP. MATERIALS ANDMETHODS:
We performed in silico analysis of 56 promoters to search for cis-cAMP (CREB) and cis-thyroid (TRE) response elements, considering UCP1, SERCA2 and glyceraldehyde 3-phosphate dehydrogenase as reference. Only regulatory regions with prior in vitro validation were selected.RESULTS:
29/56 enzymes presented potential TREs in their regulatory sequence, and some scored over 0.80 (better predictive value 1) citrate synthase, phosphoglucose isomerase, succinate dehydrogenases A/C, UCP3, UCP2, UCP4, UCP5, phosphoglycerate mutase, glyceraldehyde 3-P dehydrogenase, glucokinase, malate dehydrogenase, acyl-CoA transferase (thiolase), cytochrome a3, and lactate dehydrogenase. Moreover, some enzymes have not yet been described in the literature as genomically regulated by T3.CONCLUSION:
Our results point to other enzymes which may possibly be regulated by T3 and CREB, and speculate their joint roles in contributing to the optimal thermogenic acclimation.RESUMO
OBJETIVO:
Identificar enzimas das vias da glicólise, glicogenólise, lipólise, ciclo de Krebs, cadeia respiratória e fosforilação oxidativa possivelmente reguladas por T3 e cAMP.MATERIAIS E MÉTODOS:
Analisamos 56 genes metabólicos in silico mediante a identificação dos elementos cis de regulação gênica responsivos ao T3 e cAMP (TREs, thyroid response elements e CREs, cAMP response elements), utilizando como referência o promotor da UCP1, SERCA2 e gliceraldeído 3-fosfato desidrogenase. Selecionamos somente os promotores com estudo funcional prévio in vitro.RESULTADOS:
29/56 enzimas apresentaram TREs em suas regiões regulatórias, parte com escore > 0,80 (melhor valor preditivo 1) citrato sintase, fosfoglucose isomerase, succinato desidrogenase A/C, UCP3, UCP2, UCP4, UCP5, fosfoglicerato mutase, gliceraldeído 3-P desidrogenase, glucoquinase, malato desidrogenase, acil-CoA transferase (tiolase), citocromo a3, e lactato desidrogenase; parte desconhecida como regulada por T3.CONCLUSÃO:
Os resultados do presente estudo apontam para novos genes regulados por T3 e cAMP e, por conseguinte, sua contribuição na regulação da termogênese.Key words
Full text:
1
Collection:
01-internacional
Database:
LILACS
Main subject:
Oxidative Phosphorylation
/
Triiodothyronine
/
Cyclic AMP
/
Thermogenesis
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Arq. bras. endocrinol. metab
Journal subject:
ENDOCRINOLOGIA
/
METABOLISMO
Year:
2010
Document type:
Article
/
Project document
Affiliation country:
Brazil
Country of publication:
Brazil