Identification of candidate antigens from adult stages of Toxocara canis for the serodiagnosis of human toxocariasis
Mem. Inst. Oswaldo Cruz
; 106(2): 200-206, Mar. 2011. ilus, graf, tab
Article
in En
| LILACS
| ID: lil-583946
Responsible library:
BR1.1
ABSTRACT
In the present work, we identified adult Toxocara canis antigens through sodium dodecyl sulfate-polyacrylamide gel electrophoresis for potential use in human toxocariasis immunodiagnosis. The sensitivity and specificity of several semi-purified antigens, as well as their cross-reactivity with other parasitic infections, were assessed by IgM and IgG-enzime linked immunosorbent assay. Whilst we found that the crude extract of the parasite presented limited sensitivity, specificity and high cross-reactivity against other parasites, we identified 42, 58, 68 and 97-kDa semi-purified antigens as the most promising candidates for immunodiagnosis. Moreover, the 58 and 68-kDa antigens presented the lowest IgM cross-reactivity. When tested as a combination, a mixture of the 58 and 68-kDa antigens presented 100 percent sensitivity and specificity, as well as minor cross-reactivity. Although the combination of the 42, 58, 68 and 97-kDa antigens presented 100 percent sensitivity at a dilution of 140, the low specificity and high cross-reactivity observed suggested a limited use for diagnostic purposes. Our data suggested that the 58 and 68-kDa antigens might be most suitable for the immunodiagnosis of human toxocariasis.
Key words
Full text:
1
Collection:
01-internacional
Database:
LILACS
Main subject:
Immunoglobulin G
/
Immunoglobulin M
/
Antibodies, Helminth
/
Toxocariasis
/
Toxocara canis
/
Antigens, Helminth
Type of study:
Diagnostic_studies
Limits:
Animals
/
Female
/
Humans
/
Male
Language:
En
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
MEDICINA TROPICAL
/
PARASITOLOGIA
Year:
2011
Document type:
Article
Affiliation country:
Brazil
Country of publication:
Brazil